Noncovalent Crosslinking of SH1 and SH2 to Detect Dynamic Flexibility of the SH1 Helix
In this experiment, fluorescent N- (1-pyrenyl) iodoacetamide modified the two reactive thiols, SH1 (Cys 707) and SH2 (Cys 697) on myosin to detect SH1-SH2 a -helix melting. The excimer forming property of pyrene is well suited to monitor the dynamics of the SH1 and SH2 helix melting, since the excim...
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| Format: | Others |
| Language: | English |
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University of North Texas
2000
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| Online Access: | https://digital.library.unt.edu/ark:/67531/metadc5844/ |