Noncovalent Crosslinking of SH1 and SH2 to Detect Dynamic Flexibility of the SH1 Helix

In this experiment, fluorescent N- (1-pyrenyl) iodoacetamide modified the two reactive thiols, SH1 (Cys 707) and SH2 (Cys 697) on myosin to detect SH1-SH2 a -helix melting. The excimer forming property of pyrene is well suited to monitor the dynamics of the SH1 and SH2 helix melting, since the excim...

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Bibliographic Details
Main Author: Park, Hyunguk
Other Authors: Root, Douglas D.
Format: Others
Language:English
Published: University of North Texas 2000
Subjects:
Online Access:https://digital.library.unt.edu/ark:/67531/metadc5844/