Affinity purification of NAD+-dependent formate dehydrogenase (EC 1.2.1.2) and activity of FDH in miniature enzyme bioreactors
Formate dehydrogenase from Mycobacterium vaccae (MycFDH) was cloned and expressed from various plasmid constructs that incorporate hexahistidine tags onto the N-and C-termini of the protein. The most successful FDH variant, dual-tagged FDH L-S expressed from pET28a+, was batch-purified using ammoniu...
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| Language: | English en |
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2010
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| Online Access: | http://hdl.handle.net/1828/2489 |