Expression of human α-N-Acetylglucosaminidase in Sf9 insect cells: effect of cryptic splice site removal and native secretion-signaling peptide addition.

Expression of human α-N-Acetylglucosaminidase in Sf9 insect cells: effect of cryptic splice site removal and native secretion-signaling peptide addition.

Human α-N-Acetylglucosaminidase (Naglu) is a lysosomal acid hydrolase implicated in tthe rare metabolic storage disorder known as mucopolysaccharidosis type IIIB (MPS IIIB; also Sanfilippo syndrome B). Absence of this enzyme results in cytotoxic accumulation of heparan sulphate in the central...

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Bibliographic Details
Main Author: Jantzen, Roni Rebecca
Other Authors: Choy, Francis Y. M.
Language:English
en
Published: 2011
Subjects:
α-N-Acetylglucosaminidase
Sf9
insect cells
secretion signal peptide
lysosomal enzyme
mucopolysaccharidosis
MPS IIIB
sanfilippo syndrome
protein expression
protein transduction domain
splice site
cryptic splicing
site-directed mutagenesis
Naglu
cDNA
protein purification
hydrophobic interaction chromatography
FPLC
recombinant human protein
fusion protein
Tat-PTD
Online Access:http://hdl.handle.net/1828/3455

Internet

http://hdl.handle.net/1828/3455

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