Purification of synchronized Escherichia coli transcription elongation complexes by reversible immobilization on magnetic beads
Synchronized transcription elongation complexes (TECs) are a fundamental tool for in vitro studies of transcription and RNA folding. Transcription elongation can be synchronized by omitting one or more nucleoside triphosphates from an in vitro transcription reaction so that RNA polymerase can only t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Biochemistry and Molecular Biology Inc.
2022
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |