Heterologous expression and characterization of a thermoalkaliphilic SAM-synthetase from giant leucaena (Leucaena leucocephala subsp glabrata)

The cDNA encoding S-adenosylmethionine (SAM) synthetase was isolated from giant leucaena (Leucaena leucocephala subsp. glabrata) root tissue mRNA. Transcriptome data and 5′-RLM-RACE were used to obtain the transcript sequence and clone into the T7-expression vector pEt14b. N-terminal Histidine-tagge...

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Bibliographic Details
Main Authors: Borthakur, D. (Author), Carrillo, J.T (Author)
Format: Article
Language:English
Published: Elsevier Masson s.r.l. 2022
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