Heterologous expression and characterization of a thermoalkaliphilic SAM-synthetase from giant leucaena (Leucaena leucocephala subsp glabrata)
The cDNA encoding S-adenosylmethionine (SAM) synthetase was isolated from giant leucaena (Leucaena leucocephala subsp. glabrata) root tissue mRNA. Transcriptome data and 5′-RLM-RACE were used to obtain the transcript sequence and clone into the T7-expression vector pEt14b. N-terminal Histidine-tagge...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Masson s.r.l.
2022
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |