Generation of αMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting

The cardiac muscle-specific protein, α-myosin heavy chain (αMHC), is a major component of cardiac muscle filaments involved in cardiac muscle contraction. Here, we established an αMHC-enhanced fluorescent protein (EGFP) knock-in human pluripotent stem cell (hPSC) line by linking the EGFP gene to the...

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Bibliographic Details
Main Authors: Choi, Y.M (Author), Hwang, S. (Author), Jang, J. (Author), Jeong, S.M (Author), Jun, J.K (Author), Jung, J. (Author), Kim, A.-H (Author), Kim, H.-S (Author), Lee, H.M (Author), Seol, H. (Author), Sung, J.J (Author)
Format: Article
Language:English
Published: Elsevier B.V. 2022
Online Access:View Fulltext in Publisher