Protocol for in vitro analysis of pro-inflammatory and metabolic functions of cultured primary murine astrocytes

Robust protocols are required to investigate in vitro the molecular mechanisms that control astrocyte metabolism and pro-inflammatory activities. In the present protocol, we describe step by step the isolation and culture of primary murine astrocytes from neonatal brains, followed by their genetic m...

Full description

Bibliographic Details
Main Authors: Gutiérrez-Vázquez, C. (Author), Quintana, F.J (Author)
Format: Article
Language:English
Published: Cell Press 2022
Subjects:
Online Access:View Fulltext in Publisher