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01368nam a2200205Ia 4500 |
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10-1016-j-xpro-2021-101033 |
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220420s2022 CNT 000 0 und d |
| 020 |
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|a 26661667 (ISSN)
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| 245 |
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|a Protocol for in vitro analysis of pro-inflammatory and metabolic functions of cultured primary murine astrocytes
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| 260 |
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|b Cell Press
|c 2022
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| 856 |
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|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.xpro.2021.101033
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|a Robust protocols are required to investigate in vitro the molecular mechanisms that control astrocyte metabolism and pro-inflammatory activities. In the present protocol, we describe step by step the isolation and culture of primary murine astrocytes from neonatal brains, followed by their genetic manipulation with siRNA. We further describe cytokine activation of the cultured astrocytes for the analysis of their pro-inflammatory responses, and the oxygen consumption analysis to assess their metabolic function. For complete details on the use and execution of this protocol, please refer to Chao et al. (2019), Clark et al. (2021), and Rothhammer et al. (2018). © 2021 The Author(s)
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|a Cell culture
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|a Cell isolation
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|a Immunology
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|a Metabolism
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|a Neuroscience
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| 700 |
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|a Gutiérrez-Vázquez, C.
|e author
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|a Quintana, F.J.
|e author
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| 773 |
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|t STAR Protocols
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