Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients

Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients

Here we present an optimized protocol for transcriptome profiling of COVID-19 patient samples, including peripheral blood mononuclear cells (PBMCs) and formalin-fixed paraffin-embedded tissue samples obtained from the lung, liver, heart, kidney, and spleen, with the matched controls. We describe RNA...

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Bibliographic Details
Main Authors: Graham, J.J (Author), Kalbasi, A. (Author), Longhi, M.S (Author), Vuerich, M. (Author), Wang, N. (Author)
Format: Article
Language:English
Published: Cell Press 2022
Subjects:
adult
Adult
aged
Aged
Aged, 80 and over
autopsy
Autopsy
case control study
Case-Control Studies
Cell Biology
Cell isolation
Cell-based Assays
chemistry
Clinical Protocol
COVID-19
diagnosis
female
Female
formaldehyde
Formaldehyde
Gene Expression
genetics
Health Sciences
human
Humans
Immunology
isolation and purification
Leukocytes, Mononuclear
male
Male
metabolism
Microbiology
middle aged
Middle Aged
Molecular Biology
mononuclear cell
procedures
RNA, Viral
SARS-CoV-2
tissue fixation
Tissue Fixation
transcriptome
Transcriptome
very elderly
virology
virus RNA
young adult
Young Adult
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