Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE)

Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE)

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One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...

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Bibliographic Details
Main Authors: Luo, J. (Author), Mao, S. (Author), Ren, S. (Author), Song, P. (Author), Wang, M.X (Author), Wang, Y. (Author), Xie, N.G (Author), Yang, Y. (Author), Zhang, D.Y (Author)
Format: Article
Language:English
Published: Nature Research 2022
Subjects:
algorithm
Algorithms
DNA Primers
genetics
Likelihood Functions
multiplex polymerase chain reaction
Multiplex Polymerase Chain Reaction
primer DNA
procedures
real time polymerase chain reaction
Real-Time Polymerase Chain Reaction
statistical model
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