Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows

Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows

The global spread and continued evolution of SARS-CoV-2 has driven an unprecedented surge in viral genomic surveillance. Amplicon-based sequencing methods provide a sensitive, low-cost and rapid approach but suffer a high potential for contamination, which can undermine laboratory processes and resu...

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Bibliographic Details
Main Authors: Adams, G. (Author), Bauer, M.R (Author), Berenzy, D. (Author), Dewey, H.B (Author), Dobbins, S.T (Author), Figueroa, K. (Author), Gladden-Young, A. (Author), Hooper, D. (Author), Kales, S. (Author), Lagerborg, K.A (Author), Lemieux, J.E (Author), Loreth, C. (Author), MacInnis, B.L (Author), Normandin, E. (Author), Park, D.J (Author), Pearlman, L.R (Author), Pierce, V.M (Author), Reilly, S.K (Author), Sabeti, P.C (Author), Shaw, B.M (Author), Shenoy, E.S (Author), Siddle, K.J (Author), Tewhey, R. (Author), Zachary, K.C (Author)
Format: Article
Language:English
Published: Nature Research 2022
Subjects:
amplicon
Article
binding site
complementary DNA
controlled study
COVID-19
diagnosis
DNA contamination
DNA Primers
DNA sequence
DNA sequencing
genetics
Genome, Viral
human
Humans
metagenomics
nonhuman
primer DNA
procedures
quality control
Quality Control
reproducibility
Reproducibility of Results
RNA, Viral
SARS-CoV-2
sequence analysis
Sequence Analysis
sequence homology
Severe acute respiratory syndrome coronavirus 2
synthesis
synthetic DNA
viral contamination
virus detection
virus genome
virus RNA
virus spike
whole genome sequencing
Whole Genome Sequencing
workflow
Workflow
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