Activating cryptic biosynthetic gene cluster through a CRISPR-Cas12a-mediated direct cloning approach

Activating cryptic biosynthetic gene cluster through a CRISPR-Cas12a-mediated direct cloning approach

Direct cloning of biosynthetic gene clusters (BGCs) from microbial genomes facilitates natural product-based drug discovery. Here, by combining Cas12a and the advanced features of bacterial artificial chromosome library construction, we developed a fast yet efficient in vitro platform for directly c...

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Bibliographic Details
Main Authors: Bai, L. (Author), Karthik, L. (Author), Liang, M. (Author), Liu, J. (Author), Liu, L. (Author), Liu, X. (Author), Tan, G.-Y (Author), Tong, Y. (Author), Wang, R. (Author), Wang, S. (Author), Wang, W. (Author), Wu, M. (Author), Xu, F. (Author), Yang, J. (Author), Yang, Z. (Author), Zeng, X. (Author), Zhang, L.-X (Author), Zhu, G. (Author)
Format: Article
Language:English
Published: Oxford University Press 2022
Subjects:
biological product
Biological Products
Cloning, Molecular
CRISPR Cas system
CRISPR-Cas Systems
genetics
molecular cloning
multigene family
Multigene Family
Streptomyces
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