Expansion of the prime editing modality with Cas9 from Francisella novicida

Expansion of the prime editing modality with Cas9 from Francisella novicida

Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse...

Full description

Bibliographic Details
Main Authors: Gwon, L.W (Author), Huh, J.-W (Author), Hur, J.K (Author), Jun, B.-H (Author), Jung, C. (Author), Kim, C.H (Author), Kim, H. (Author), Kim, S.-U (Author), Kim, Y.-H (Author), Lee, S.H (Author), Lee, W.-J (Author), Lee, Y. (Author), Lim, K.-S (Author), Oh, Y. (Author), Park, Y.-H (Author), Song, B.-S (Author), Song, W.J (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2022
Subjects:
CRISPR Cas system
CRISPR-Cas Systems
CRISPR-Cas9
deoxyribonuclease I
Deoxyribonuclease I
Francisella
Francisella novicida
gene editing
Gene Editing
human
Humans
metabolism
Ortholog
Prime editing
RNA directed DNA polymerase
RNA-Directed DNA Polymerase
Target expansion
Online Access:View Fulltext in Publisher

Internet

View Fulltext in Publisher

Similar Items