Identification and Tracking of Alloreactive T Cell Clones in Rhesus Macaques Through the RM-scTCR-Seq Platform

T cell receptor (TCR) clonotype tracking is a powerful tool for interrogating T cell mediated immune processes. New methods to pair a single cell’s transcriptional program with its TCR identity allow monitoring of T cell clonotype-specific transcriptional dynamics. While these technologies have been...

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Bibliographic Details
Main Authors:	Cagnin, L. (Author), Fleming, R.A (Author), Gerdemann, U. (Author), Kaminski, J. (Author), Kean, L.S (Author), Keskula, P. (Author), Lane, J.F (Author), McGuckin, C. (Author), Rui, X. (Author), Shalek, A.K (Author), Tkachev, V. (Author)
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022
Subjects:	alloreactive T cells animal animal cell animal experiment animal model Animals Article CD8+ T lymphocyte cell clone cell tracking Cell Tracking cellular immunity controlled study cryopreservation cytotoxic T lymphocyte DNA extraction epitope flow cytometry gene amplification gene expression gene expression profiling Gene Expression Profiling gene expression regulation Gene Expression Regulation gene mutation genetic transcription genetics graft versus host reaction GVHD high throughput sequencing High-Throughput Nucleotide Sequencing immunology in vitro study interleukin 2 long untranslated RNA lymphocyte activation Lymphocyte Activation lymphocyte antigen receptor Lymphocyte Culture Test, Mixed Macaca mulatta metabolism microarray analysis mitochondrial DNA mixed lymphocyte culture mixed lymphocyte reaction nested polymerase chain reaction nonhuman peripheral blood mononuclear cell procedures quality control Receptors, Antigen, T-Cell regulatory T lymphocyte Rhesus Macaque (Macaca mulatta) rhesus monkey RNA sequence Sanger sequencing sequence analysis single cell sequencing T lymphocyte receptor T lymphocyte subpopulation target organ TCR sequencing Th17 cell T-Lymphocyte Subsets transcriptome Transcriptome upregulation whole exome sequencing
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Identification and Tracking of Alloreactive T Cell Clones in Rhesus Macaques Through the RM-scTCR-Seq Platform

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