Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C

Aberrant remodeling of the asthmatic airway is not well understood but is thought to be attributable in part to mechanical compression of airway epithelial cells. Here, we examine compression-induced expression and secretion of the extracellular matrix protein tenascin C (TNC) from well-differentiat...

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Bibliographic Details
Main Authors:	De Marzio, M. (Author), Fredberg, J.J (Author), Kılıç, A. (Author), Mitchel, J.A (Author), Mwase, C. (Author), O’sullivan, M.J (Author), Park, J.-A (Author), Phung, T.-K.N (Author), Weiss, S.T (Author)
Format:	Article
Language:	English
Published:	MDPI 2022
Subjects:	1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene 33352 4 [4 (1,3 benzodioxol 5 yl) 5 (2 pyridinyl) 1h imidazol 2 yl]benzamide 5174s af2339 Airway epithelial cells airway epithelium cell airway remodeling Airway remodeling Article asthma Asthma bovine serum albumin bronchospasm Bronchospasm carbon dioxide cc065 CD9 antigen cell culture centrifugation complementary DNA compressive strength Compressive Strength cytology dimethyl sulfoxide disease exacerbation dithiothreitol enzyme linked immunosorbent assay Epithelial Cells epithelium cell exosome extracellular matrix Extracellular matrix Extracellular vesicles Extracellular Vesicles gene expression genetics glyceraldehyde 3 phosphate dehydrogenase growth factor HBEC cell line (bronchial epithelium) human Humans incubation time lung Lung MAP Kinase Signaling System MAPK signaling Mechanical compression mechanical stress messenger RNA metabolism mitogen activated protein kinase mitogen activated protein kinase kinase inhibitor mRNA expression level nonhuman nystatin phenotype real time polymerase chain reaction Receptors, Transforming Growth Factor beta retinoic acid RNA directed DNA polymerase RNA isolation RNA sequence RNA, Messenger Stress, Mechanical tenascin Tenascin Tenascin C TGF beta signaling thromboplastin transferrin transforming growth factor beta transforming growth factor beta receptor water Western blotting
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Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C

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