Mapping Interactions between Glycans and Glycan-Binding Proteins by Live Cell Proximity Tagging

Mapping Interactions between Glycans and Glycan-Binding Proteins by Live Cell Proximity Tagging

Interactions between glycans and glycan-binding proteins (GBPs) consist of weak, noncovalent, and transient binding events, making them difficult to study in live cells void of a static, isolated system. Furthermore, the glycans are often presented as protein glycoconjugates, but there are limited e...

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Bibliographic Details
Main Authors: Huang, M.L (Author), Joeh, E. (Author), Parker, C.G (Author), Reeves, A.E (Author)
Format: Article
Language:English
Published: Blackwell Publishing Inc. 2021
Subjects:
alkylation
Article
binding affinity
carrier protein
Carrier Proteins
carrier proteins and binding proteins
cells
chemical labeling
enzyme linked immunosorbent assay
fluorescence microscopy
fusion protein
galectin
galectin 3
glycan
glycan binding protein
glycoprotein
Glycoproteins
interactomics
mass spectrometry
Mass Spectrometry
peroxidase
plasmid
polysaccharide
Polysaccharides
protein analysis
protein binding
protein denaturation
protein expression
protein interaction
protein modification
protein purification
proteomics
Proteomics
proximity labeling
quantitative analysis
radical
recombinant protein
reduction (chemistry)
standardization
streptavidin
TMT labeling
trypsinization
unclassified drug
Western blotting
workflow
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