Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast
Here we describe an updated TAR cloning protocol for the selective and efficient isolation of any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The method exploits the special recombination machinery of the yeast Saccharomyces cerevisiae. TAR cloning is based on the hig...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
John Wiley and Sons Inc
2021
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Subjects: | |
Online Access: | View Fulltext in Publisher |