Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast

Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast

Here we describe an updated TAR cloning protocol for the selective and efficient isolation of any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The method exploits the special recombination machinery of the yeast Saccharomyces cerevisiae. TAR cloning is based on the hig...

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Bibliographic Details
Main Authors: Kim, J.-H (Author), Kouprina, N. (Author), Larionov, V. (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
Agrobacterium tumefaciens
Article
bacterial cell
bioinformatics
chemical reaction kinetics
chromosome
Chromosomes, Artificial, Yeast
Cloning, Molecular
clustered regularly interspaced short palindromic repeat
comparative genomics
controlled study
CRISPR associated endonuclease Cas9
CRISPR Cas system
CRISPR/Cas9
CRISPR-Cas Systems
CRISPR-CAS9 system
DNA extraction
DNA fragment
DNA isolation
DNA replication origin
DNA sequence
DNA strand breakage
double stranded DNA break
electroporation
endonuclease
enzyme activity
Escherichia coli
exportin 1
gene editing
gene isolation
gene locus
gene therapy
gene vector
genetic recombination
Genetic Vectors
genetics
genome
genomic DNA
genomic fragment
genomics
Genomics
haplotype
homologous recombination
human
Humans
molecular cloning
multigene family
mutational analysis
nonhuman
nuclease
polymerase chain reaction
population research
protein purification
proteome
Recombination, Genetic
Saccharomyces cerevisiae
spheroplast
strategic planning
Streptococcus pyogenes
structure activity relation
TAR cloning
transformation-associated recombination
whole genome sequencing
yeast
yeast artificial chromosome
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