Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry

Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry

Technological advancements in fluorescence flow cytometry and an ever-expanding understanding of the complexity of the immune system have led to the development of large flow cytometry panels reaching up to 43 colors at the single-cell level. However, as panel size and complexity increase, so too do...

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Bibliographic Details
Main Authors: Ferrer-Font, L. (Author), Jaimes, M.C (Author), Johnston, L.K (Author), Lannigan, J. (Author), Lewer, B. (Author), Park, L.M (Author), Pilkington, K.R (Author), Price, K.M (Author), Small, S.J (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
antibody titer
article
assay optimization and troubleshooting
autofluorescence
data quality
dimensionality reduction
flow cytometry
Flow Cytometry
fluorescent dye
Fluorescent Dyes
full-spectrum flow cytometry
high-dimensional flow cytometry panel
human
human cell
human tissue
Humans
immunophenotyping
Immunophenotyping
laser
Lasers
medical research
T lymphocyte
T lymphocyte subpopulation
T-Lymphocyte Subsets
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