Generating CRISPR-Cas9-Mediated Null Mutations and Screening Targeting Efficiency in Human Pluripotent Stem Cells

Generating CRISPR-Cas9-Mediated Null Mutations and Screening Targeting Efficiency in Human Pluripotent Stem Cells

CRISPR-Cas9 mutagenesis facilitates the investigation of gene function in a number of developmental and cellular contexts. Human pluripotent stem cells (hPSCs), either embryonic or induced, are a tractable cellular model to investigate molecular mechanisms involved in early human development and cel...

Full description

Bibliographic Details
Main Authors: Alanis-Lobato, G. (Author), Bower, O.J (Author), Gerri, C. (Author), Lea, R.A (Author), McCarthy, A. (Author), Niakan, K.K (Author), Turner, J.M.A (Author), Zohren, J. (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
Article
bioinformatics
cell cloning
cell differentiation
cell viability
clonal derivation
colony formation
controlled study
copy number variation
CRISPR Cas system
CRISPR-Cas Systems
CRISPR-Cas9
CRISPR-CAS9 system
DNA extraction
DNA sequence
frameshift mutation
gene amplification
gene editing
Gene Editing
gene expression
gene function
gene mutation
gene sequence
genetic screening
genetic transcription
genetic transfection
genetics
genome editing
genome size
guide RNA
human
human pluripotent stem cells
Humans
indel mutation
loss of function mutation
Loss of Function Mutation
mutagenesis
nonhuman
null allele
plasmid
pluripotent stem cell
Pluripotent Stem Cells
polymerase chain reaction
prevalence
protein phosphorylation
protein structure
quality control
RNA extraction
RNA sequence
RNA, Guide
Sanger sequencing
sgRNA efficiency
stem cell line
Streptococcus pyogenes
whole exome sequencing
Online Access:View Fulltext in Publisher

Internet

View Fulltext in Publisher

Similar Items