Determining ADCC Activity of Antibody-Based Therapeutic Molecules using Two Bioluminescent Reporter-Based Bioassays

Determining ADCC Activity of Antibody-Based Therapeutic Molecules using Two Bioluminescent Reporter-Based Bioassays

Antibody Fc effector function is one of the main mechanisms of action (MoA) for therapeutic monoclonal antibodies. Measurement of antibody-dependent cellular cytotoxicity (ADCC) is critical for understanding the Fc effector function during monoclonal antibody development. This article covers two cel...

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Bibliographic Details
Main Authors: Cheng, Z.J (Author), Cong, M. (Author), Fan, F. (Author), Garvin, D. (Author), Gilden, J. (Author), Grailer, J. (Author), Hartnett, J. (Author), Stecha, P. (Author), Wang, J. (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
ADCC
ADCC reporter bioassay
Antibodies, Monoclonal
antibody dependent cellular cytotoxicity
Antibody-Dependent Cell Cytotoxicity
Article
bioassay
Biological Assay
bioluminescence
cell assay
controlled study
cytolysis
drug binding
drug potency
effector cell
Fc function
Fc receptor
HiBiT target cells
human
human cell
Killer Cells, Natural
Leukocytes, Mononuclear
luciferase
materials handling
monoclonal antibody
mononuclear cell
natural killer cell
peripheral blood mononuclear cell
primary PBMC
process optimization
quantitative analysis
rituximab
target cell
trastuzumab
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