Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Class II major histocompatibility complex peptide (MHC-IIp) multimers are precisely engineered reagents used to detect T cells specific for antigens from pathogens, tumors, and self-proteins. While the related Class I MHC/peptide (MHC-Ip) multimers are usually produced from subunits expressed in E....

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Bibliographic Details
Main Authors: Altman, J.D (Author), Bai, G. (Author), Bell, D.L (Author), Contreras-Alcantara, S. (Author), Han, L. (Author), Jeter, K. (Author), Kazarian, T. (Author), Laur, O. (Author), Long, D.L (Author), Ramachandiran, V. (Author), Shires, J.C (Author), Ugwu, K.C (Author), Willis, R.A (Author)
Format: Article
Language:English
Published: Blackwell Publishing Inc. 2021
Subjects:
allele
amino terminal sequence
animal cell
antigen-specific T cells
Article
beta chain
biotinylation
catalysis
Class II MHC multimers
Class II MHC tetramers
controlled study
dyes, reagents, indicators, markers and buffers
elution
Escherichia coli
flow cytometry
gel electrophoresis
gene expression system
HEK293 cell line
HEK293 Cells
HEK293T cell line
high throughput screening
Histocompatibility Antigens Class II
HLA antigen class 2
HLA DM antigen
human
human cell
Humans
Indicators and Reagents
isoelectric focusing
lentiviral transduction
Lentivirus
lentivirus vector
ligand
major histocompatibility antigen class 2
matrix assisted laser desorption ionization time of flight mass spectrometry
mouse
nonhuman
priority journal
protein cleavage
protein engineering
protein expression
protein multimerization
protein purification
proteinase
reagent
staining
Staining and Labeling
T lymphocyte
tetramer
viral gene delivery system
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