Silencing of the CrkL gene reverses the doxorubicin resistance of K562/ADR cells through regulating PI3K/Akt/MRP1 signaling

Background: Doxorubicin is a first-line chemotherapy agent on human myelogenous leukemia clinical treatment, but the development of chemoresistance has largely limited curative effect. In this study, we aimed to evaluate the biological function and molecular mechanisms of CrkL to Doxorubicin resista...

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Bibliographic Details
Main Authors:	Chen, R.-W (Author), Chen, W.-X (Author), Lin, D.-H (Author), Lin, D.-Q (Author), Xie, W.-J (Author), Xu, J.-P (Author), Ye, W.-L (Author), You, W.-W (Author), Yu, J. (Author), Zhang, H.-Q (Author)
Format:	Article
Language:	English
Published:	John Wiley and Sons Inc 2021
Subjects:	3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide Adaptor Proteins, Signal Transducing animal animal cell animal experiment animal model Animals Antibiotics, Antineoplastic antineoplastic antibiotic apoptosis Apoptosis Article cancer inhibition cell proliferation controlled study Crk like protein CrkL CrkL gene doxorubicin Doxorubicin doxorubicin resistance drug effect drug efficacy drug resistance Drug Resistance, Neoplasm drug screening drug sensitivity flow cytometry gene expression gene expression regulation Gene Expression Regulation, Neoplastic gene function gene overexpression gene silencing Gene Silencing genetic transfection genetics human human cell Humans IC50 in vivo study K-562 cell line K562 Cells K562/Adr cell line metabolism Mice, Nude molecular biology mouse MRP1 MRP1 gene multidrug resistance associated protein multidrug resistance associated protein 1 multidrug resistance-associated protein 1 Multidrug Resistance-Associated Proteins myelogenous leukemia myeloid leukemia nonhuman nude mouse phosphatidylinositol 3 kinase Phosphatidylinositol 3-Kinases PI3K Akt MRP1 signaling pathway protein expression protein kinase B Proto-Oncogene Proteins c-akt real time reverse transcription polymerase chain reaction RNA interference signal transducing adaptor protein signal transduction Signal Transduction small interfering RNA tumor growth tumor xenograft upregulation Western blotting Xenograft Model Antitumor Assays
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Silencing of the CrkL gene reverses the doxorubicin resistance of K562/ADR cells through regulating PI3K/Akt/MRP1 signaling

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