Cross-platform comparison of next-generation sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for detecting KRAS/NRAS/BRAF/PIK3CA mutations in cfDNA from metastatic colorectal cancer patients

Cross-platform comparison of next-generation sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for detecting KRAS/NRAS/BRAF/PIK3CA mutations in cfDNA from metastatic colorectal cancer patients

Background: Examining tumor KRAS/NRAS/BRAF/PIK3CA status in metastatic colorectal cancer (mCRC) is essential for treatment selection and prognosis evaluation. Cell-free DNA (cfDNA) in plasma is a feasible source for tumor gene analysis. Methods: In this study, we recruited mCRC patients and analyzed...

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Bibliographic Details
Main Authors: Cao, M. (Author), Chen, X. (Author), Guo, W. (Author), Huang, F. (Author), Jiang, H. (Author), Liu, T. (Author), Shen, M. (Author), Wang, B. (Author), Wang, H. (Author), Xu, X. (Author), Yang, Y. (Author), Yu, Y. (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
adult
aged
Aged
Article
B Raf kinase
Biomarkers, Tumor
BRAF protein, human
CA 19-9 antigen
cancer patient
carcinoembryonic antigen
cell free nucleic acid
cell-free DNA
Cell-Free Nucleic Acids
circulating tumor DNA
Class I Phosphatidylinositol 3-Kinases
Colorectal Neoplasms
colorectal tumor
comparative study
correlational study
disease burden
disease exacerbation
female
Female
follow up
Follow-Up Studies
gene frequency
gene mutation
genetic analysis
genetic correlation
genetics
GTP Phosphohydrolases
guanosine triphosphatase
high throughput sequencing
High-Throughput Nucleotide Sequencing
human
Humans
KRAS protein, human
Liver Neoplasms
liver tumor
Lung Neoplasms
lung tumor
major clinical study
male
Male
matrix assisted laser desorption ionization time of flight mass spectrometry
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
matrix-assisted laser desorption-ionization mass spectrometry
membrane protein
Membrane Proteins
metastatic colorectal cancer
middle aged
Middle Aged
mutation
Mutation
next-generation sequencing
NRAS protein, human
pathology
patient monitoring
phosphatidylinositol 4,5 bisphosphate 3 kinase
PIK3CA protein, human
predictive value
procedures
prognosis
Prognosis
Prospective Studies
prospective study
protein p21
Proto-Oncogene Proteins B-raf
Proto-Oncogene Proteins p21(ras)
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
tumor marker
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