Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types

Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types

Background: Liquid biopsy is gaining increasing popularity in cancer screening and diagnosis. However, there is no relatively mature DNA isolation method or commercial kit available that is compatible with different LB sample types. This study developed a PAN-sample DNA isolation method (PAN method)...

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Bibliographic Details
Main Authors: Cao, S.-M (Author), Chen, W. (Author), Chen, W.-J (Author), Chen, X.-X (Author), Li, M. (Author), Lin, C.-X (Author), Lin, D.-F (Author), Liu, Y.-L (Author), Xie, S.-H (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
16S rDNA gene
adult
Article
blood analysis
case control study
Case-Control Studies
clinical article
complication
concentration (parameter)
controlled study
DNA extraction
DNA extraction method
DNA isolation
DNA methylation
DNA Methylation
DNA purification
DNA sequencing
DNA, Viral
Epstein Barr virus
Epstein Barr virus infection
Epstein-Barr Virus Infections
feces
Feces
feces analysis
gene mutation
genetics
guanidine thiocyanate
Herpesvirus 4, Human
HLA DP antigen
HLA DPB1 gene
HLA-DP beta-Chains
HLA-DPB1 antigen
human
Humans
isolation and purification
liquid biopsy
Liquid Biopsy
mutational analysis
Nasopharyngeal Neoplasms
nasopharyngeal swab
nasopharynx carcinoma
nasopharynx tumor
next-generation sequencing (NGS)
nonhuman
oncogene K ras
polymerase chain reaction
Polymerase Chain Reaction
procedures
process development
Q-PCR
RNA 16S
RNA, Ribosomal, 16S
saliva
Saliva
saliva analysis
sanger sequencing (SS)
specimen handling
Specimen Handling
thiocyanate
tumor-related gene
unclassified drug
validation process
virology
virus DNA
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