Economic and ecological benefits of a leaky E. coli strain for downstream processing: a case study for staphylococcal protein A

Economic and ecological benefits of a leaky E. coli strain for downstream processing: a case study for staphylococcal protein A

BACKGROUND: Downstream processing of soluble recombinant proteins from Escherichia coli is complicated by the need to access the intracellular product by cell disruption and to separate the target protein from impurities, particularly host cell protein, DNA, endotoxins and lipids. We previously demo...

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Bibliographic Details
Main Authors: Cataldo, A.L (Author), Ebner, J. (Author), Jungbauer, A. (Author), Kastenhofer, J. (Author), Sedlmayr, V.L (Author), Spadiut, O. (Author)
Format: Article
Language:English
Published: John Wiley and Sons Ltd 2021
Subjects:
amino acid sequence
anion exchange
anion exchange chromatography
Anion exchange chromatography
Article
bacterial growth
bacterial outer membrane
bevifimod
capture chromatography
carbon dioxide
carbon footprint
cell disruption
cell lysate
Chemical industry
Chromatography
conductance
cost
cost of goods sold
Cost reduction
dissolved oxygen
downstream processing
Ecology
Economic analysis
Economic and social effects
Environmental footprints
Escherichia coli
Escherichia coli endotoxin
Extracellular proteins
hyperbarism
Industrial economics
Intracellular production
Intracellular products
Ion exchangers
limit of detection
nonhuman
Presses (machine tools)
primary recovery
process mass intensity
process model
protein secretion
Recombinant protein productions
Recombinant proteins
Recovery
reversed phase high performance liquid chromatography
soluble protein expression
Staphylococcal protein
Staphylococcal protein a
temperature
water
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