Lipid reduction to improve clarification and filterability during primary recovery of intracellular products in yeast lysates using exogenous lipase

Lipid reduction to improve clarification and filterability during primary recovery of intracellular products in yeast lysates using exogenous lipase

BACKGROUND: The yeast Pichia pastoris is a popular host organism for production of a range of biological products, several of which are intracellular. The disruption of yeast cells by homogenization also releases large quantities of lipids, which can foul the downstream membranes and chromatography...

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Bibliographic Details
Main Authors: Bandyopadhyay, S.K (Author), Bracewell, D.G (Author), Morris, S.A (Author), Nesbeth, D.N (Author)
Format: Article
Language:English
Published: John Wiley and Sons Ltd 2021
Subjects:
Article
Biological Products
bioprocessing
cell free system
cell lysate
centrifugation
Centrifugation
Chemical industry
Chemical technologies
Chromatography
Clarification
Clarifiers
enzymatic degradation
Enzymatic Degradation
Enzymatic digestions
filtration
Filtration performance
Intracellular products
Komagataella pastoris
lipid
Lipids
Microfiltration
nonhuman
nuclease
polyethylene
primary recovery
Process Improvement
proof of concept
Proof of concept
sulfone
temperature
triacylglycerol lipase
Yeast
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