Simple method of thawing cryo-stored samples preserves ultrastructural features in electron microscopy

Simple method of thawing cryo-stored samples preserves ultrastructural features in electron microscopy

Preservation of ultrastructural features in biological samples for electron microscopy (EM) is a challenging task that is routinely accomplished through chemical fixation or high-pressure freezing coupled to automated freeze substitution (AFS) using specialized devices. However, samples from clinica...

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Bibliographic Details
Main Authors: Dohr, G. (Author), Galhuber, M. (Author), Gauster, M. (Author), Gschwandtner, E. (Author), Jandl, K. (Author), Kolb, D. (Author), Kratky, D. (Author), Kupper, N. (Author), Kwapiszewska, G. (Author), Leitinger, G. (Author), Olschewski, A. (Author), Prokesch, A. (Author), Schweiger, M. (Author)
Format: Article
Language:English
Published: Springer Science and Business Media Deutschland GmbH 2021
Subjects:
animal
animal tissue
Animals
Article
Biobanking
brown adipose tissue
C57BL mouse
cardiac muscle
controlled study
cryopreservation
Cryopreservation
Cryoprotectant-free
Cryo-storage
electron microscopy
fat droplet
freezing
Freezing
human
human tissue
Lipid Droplets
liver
Liver
Mice
Mice, Inbred C57BL
Microscopy, Electron
Mitochondria
mitochondrion
morphometry
mouse
nonhuman
placenta
priority journal
Sample preparation
skeletal muscle
TEM
thawing
Ultrastructural features
ultrastructure
white adipose tissue
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