A rapid and robust method for the cryopreservation of human granulosa cells

A rapid and robust method for the cryopreservation of human granulosa cells

Human primary granulosa cells (GCs) derived from women undergoing oocyte retrieval can be cultured and used as a cellular model for the study of human ovarian function. In vitro, they change rapidly, initially resembling cells of the preovulatory follicle and then cells of the corpus luteum. They ar...

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Bibliographic Details
Main Authors: Berg, D. (Author), Berg, U. (Author), Beschta, S. (Author), Bohne, N. (Author), Eubler, K. (Author), Forne, I. (Author), Mayerhofer, A. (Author)
Format: Article
Language:English
Published: Springer Science and Business Media Deutschland GmbH 2021
Subjects:
adult
aromatase
Article
cell contact
cell count
cell culture
Cell culture
cell isolation
cell structure
cell survival
cell survival rate
Cells, Cultured
cholesterol monooxygenase (side chain cleaving)
controlled study
cryopreservation
Cryopreservation
cytology
false discovery rate
female
Female
granulosa cell
Granulosa Cells
human
human cell
Humans
IVF
mass spectrometry
Mass spectrometry
medical history
mRNA expression level
Ovarian cells
ovary follicle fluid
primary cell
progesterone
Progesterone
proteomics
RNA isolation
steroidogenesis
survival rate
thawing
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