Directed evolution of the PobR allosteric transcription factor to generate a biosensor for 4-hydroxymandelic acid

Directed evolution of the PobR allosteric transcription factor to generate a biosensor for 4-hydroxymandelic acid

Hydroxy-mandelic acid (HMA) is widely applied in pharmaceuticals, food and cosmetics. In this study, we aimed to develop an allosteric transcription factors (aTFs) based biosensor for HMA. PobR, an aTF for HMA analog 4-hydroxybenzoic acid, was used to alter its selectivity and create novel aTFs resp...

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Bibliographic Details
Main Authors: Chen, F. (Author), Dong, Y. (Author), Fu, H. (Author), Guo, S. (Author), Liang, Y.Y (Author), Luo, J. (Author), Su, K. (Author), Sui, G. (Author), Wang, P. (Author), Wang, Z. (Author), Yang, C. (Author), Zhang, B. (Author), Zhang, Y. (Author)
Format: Article
Language:English
Published: Springer Science and Business Media B.V. 2022
Subjects:
4-hydroxybenzoic acids
Acid production
Biosensors
Directed evolution
Error prone PCR
Hydroxymandelic acid
Hydroxy-mandelic acid
Ligand specificity
Ligands
Mandelic acid
Mutant libraries
Organic acids
Pobr
PobR
Transcription
Transcription factors
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