Assessment of static and perfusion methods for decellularization of PCL membrane-supported periodontal ligament cell sheet constructs

Assessment of static and perfusion methods for decellularization of PCL membrane-supported periodontal ligament cell sheet constructs

Objectives: Decellularization aims to harness the regenerative properties of native extracellular matrix. The objective of this study was to evaluate different methods of decellularization of periodontal ligament cell sheets whilst maintaining their structural and biological integrity. Design: Human...

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Bibliographic Details
Main Authors: Farag, A. (Author), Hashimi, S.M (Author), Hutmacher, D.W (Author), Ivanovski, S. (Author), Vaquette, C. (Author), Volpato, F.Z (Author)
Format: Article
Language:English
Published: Elsevier Ltd 2018
Subjects:
acellular dermal matrix
Acellular Dermis
ammonium hydroxide
Ammonium Hydroxide
cell culture technique
Cell Culture Techniques
cell proliferation
Cell Proliferation
Cell sheet
cell size
Cell Size
collagen
Collagen
cytology
Decellularization
deoxyribonuclease
Deoxyribonucleases
DNA
dodecyl sulfate sodium
extracellular matrix
Extracellular Matrix
fibroblast growth factor
Fibroblast Growth Factors
Guided Tissue Regeneration, Periodontal
Hepatocyte Growth Factor
HGF protein, human
human
Humans
Melt electrospinning
metabolism
octoxinol
Octoxynol
periodontal guided tissue regeneration
periodontal ligament
Periodontal ligament
Periodontal Ligament
polycaprolactone
Polycaprolactone
polyester
Polyesters
procedures
scatter factor
Sodium Dodecyl Sulfate
tissue engineering
Tissue engineering
Tissue Engineering
tissue scaffold
Tissue Scaffolds
Vascular Endothelial Growth Factor A
vasculotropin A
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