Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II® CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia

Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II® CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia

This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives...

Full description

Bibliographic Details
Main Authors: Abdullah, U.Y.H (Author), Fakhruzzaman bin Noorizhab, M.N (Author), Fucharoen, S. (Author), Ibrahim, H.M (Author), Jassim, H.M (Author), Kek, T.L (Author), Salleh, M.Z (Author), Wilairat, P. (Author), Zilfalil, B.A (Author)
Format: Article
Language:English
Published: Elsevier B.V. 2019
Subjects:
Anaemia
Article
beta thalassemia
calibration
controlled study
cross-sectional study
disease severity
erythrocyte
genetic variability
Globin chain
globin synthesis
hemoglobin alpha chain
hemoglobin beta chain
hemoglobin delta chain
hemoglobin gamma chain
high performance liquid chromatography
HPLC
human
human cell
ion exchange chromatography
liquid chromatography-mass spectrometry
low income country
major clinical study
Malaysia
mass spectrometry
Mass spectrometry
phenotype
phenotypic variation
proteome
proteomics
quadrupole mass spectrometry
Quantification
regulatory mechanism
Thalassemia
Unknown peaks
Online Access:View Fulltext in Publisher
View in Scopus

Internet

View Fulltext in Publisher
View in Scopus

Similar Items