A new FTIR assay for quantitative measurement of endo-fucoidanase activity

A new FTIR assay for quantitative measurement of endo-fucoidanase activity

Endo-fucoidanases, including EC 3.2.1.211 endo-α-1,3-L-fucanase and EC 3.2.1.212 endo-α-1,4-L-fucanase activities, catalyze depolymerization of fucoidans – a group of bioactive, sulfated fucosyl-polysaccharides found primarily in brown macroalgae (brown seaweeds). Quantitative assessment of endo-fuc...

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Bibliographic Details
Main Authors: Baum, A. (Author), Meyer, A.S (Author), Mikkelsen, M.D (Author), Perna, V. (Author), Thi Dieu Trang, V. (Author), Thi Nguyen, T. (Author), Thi Thanh Van, T. (Author), Thi Thuy Cao, H. (Author), Tran, V.H.N (Author)
Format: Article
Language:English
Published: Elsevier Inc. 2022
Subjects:
Activity assays
Electrophoresis
Endo-fucoidanase
Enzymatic activities
Enzymatic activity assay
Enzymes
Fcna
FcnA
FFA2
Fhf1
Fourier transform infrared spectroscopy
Fourier transform infrared spectroscopy-parallel factor analyse
FTIR-PARAFAC
Fucoidanase
Light absorption
Parallel factor analysis
Sodium chloride
Stretching
Substrates
Sulfur compounds
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