pH triggered and charge attracted nanogel for simultaneous evaluation of penetration and toxicity against skin cancer: In-vitro and ex-vivo study

• Main Authors: Agrawal, R.K (Author), Iyer, A.K (Author), Jain, S. (Author), Kashaw, S.K (Author), Kushwah, V. (Author), Sahu, P. (Author), Sau, S. (Author)
• Format: Article
• Language: English
• Published: Elsevier B.V. 2019
• Subjects: acidity; animal tissue; antineoplastic agent; Antineoplastic Agents; apoptosis; Apoptosis; Article; biocompatibility; capecitabine; Capecitabine; cell line; Cell Line; chemistry; chitosan; Chitosan; controlled study; diethylene glycol monoethyl ether; diffusion; Diffusion; drug carrier; Drug Carriers; drug cytotoxicity; drug diffusion; drug effect; Drug Liberation; drug penetration; drug release; drug retention; ethylene glycol derivative; Ethylene Glycols; ex vivo study; gel; gelation; Gels; HaCat cell line; human; human cell; Humans; Hydrogen-Ion Concentration; in vitro study; metabolism; nanoencapsulation; nanogel; Nanogel; nanomaterial; Nanostructures; nonhuman; particle size; Particle Size; permeability; Permeability; pH; pig; poloxamer; Poloxamer; skin; Skin; skin cancer; Skin cancer; Skin Neoplasms; skin penetration; skin tumor; steady state; Surface Properties; surface property; zeta potential
• Online Access: View Fulltext in Publisher
• ISBN: 01418130 (ISSN)
• DOI: 10.1016/j.ijbiomac.2019.01.147

The current research is focused to develop and investigate the toxicity and penetration potential of biocompatible chitosan nanogel encapsulating capecitabine by ionic interaction mechanism exhibiting pH triggered transdermal targeting. The nanogel (CPNL) was synthesized by ion gelation mechanism using Pluronic F 127 and surface decoration by Transcutol as non-ionic penetration enhancer. The CPNL possesses fine morphology and nano size range when evaluated by TEM, SEM and DLS analysis with cationic charge and slightly acidic pH assayed by zeta potential and pH analysis. It showed pH responsive drug release characteristics mimicking the skin cancer micro-environment. The MTT assay and apoptotic index of CPNL on HaCaT cell line elaborated optimal cell toxicity and retention on 24 h of exposure. The ex-vivo skin penetration analysis exhibited noteworthy diffusion and penetration caliber through concentration depth profile, steady state flux and fluorescent skin imaging on porcine tissue. Overall outcomes suggested CPNL as a potent alternative biocompatible, transdermal nanotherapy against skin cancer displaying significant penetration caliber with enhance toxicity on cancerous cell. © 2019 Elsevier B.V.