New insights into the specificity and processivity of two novel pectinases from Verticillium dahliae

New insights into the specificity and processivity of two novel pectinases from Verticillium dahliae

Pectin, the major non-cellulosic component of primary cell wall can be degraded by polygalacturonases (PGs) and pectin methylesterases (PMEs) during pathogen attack on plants. We characterized two novel enzymes, VdPG2 and VdPME1, from the fungal plant pathogen Verticillium dahliae. VdPME1 was most a...

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Bibliographic Details
Main Authors: Bassard, S. (Author), Cherkaoui, M. (Author), Habrylo, O. (Author), Lecomte, S. (Author), Mercadante, D. (Author), Pau-Roblot, C. (Author), Pelloux, J. (Author), Pilard, S. (Author), Safran, J. (Author), Sénéchal, F. (Author), Voxeur, A. (Author)
Format: Article
Language:English
Published: Elsevier B.V. 2021
Subjects:
Article
Ascomycetes
Ascomycota
biochemical analysis
carboxylesterase
Carboxylic Ester Hydrolases
chemistry
electrospray mass spectrometry
enzyme activity
enzyme analysis
enzyme purification
enzyme release
enzyme specificity
enzyme structure
enzyme substrate
enzymology
flax
Flax
fungal cell wall
fungal protein
Fungal Proteins
fungal strain
fungus growth
genetics
heterologous expression
kinetics
Kinetics
liquid chromatography-mass spectrometry
metabolism
microbiology
Models, Molecular
molecular model
nonhuman
oligogalacturonide
Oligogalacturonides
oligomer
pathogenicity
pectin
Pectin
Pectin methylesterase
pectinesterase
Pectins
pH
phylogeny
Phylogeny
plant defense
plant disease
Plant Diseases
plant pathogen interaction
plant root
Plant Roots
polygalacturonase
Polygalacturonase
polygalacturonic acid
protein expression
sequence analysis
sequence homology
size exclusion chromatography
sodium chloride
static electricity
Static Electricity
structure analysis
Substrate Specificity
temperature
unclassified drug
Verticillium dahliae
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