Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections

Photo-isolation chemistry (PIC) enables isolation of transcriptome information from locally defined areas by photo-irradiation. Here, we present an optimized PIC protocol for formalin-fixed frozen and paraffin mouse sections and fresh-frozen mouse sections. We describe tissue section preparation and...

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Bibliographic Details
Main Authors: Harada, A. (Author), Honda, M. (Author), Kimura, R. (Author), Maehara, K. (Author), Ohkawa, Y. (Author), Oki, S. (Author), Tanaka, K. (Author)
Format: Article
Language:English
Published: Cell Press 2022
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