A live-imaging protocol for tracking receptor dynamics in single cells

Adjacent membrane receptors can show different cellular responses to ligand stimulation. Here, we describe a super-resolution microscopy imaging protocol for tracking the dynamics of two different membrane-bound receptors in single cells. We describe the transfection protocol by electroporation. We...

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Bibliographic Details
Main Authors: Gaisano, H. (Author), Huang, Y. (Author), Rakugi, H. (Author), Takahashi, T. (Author), Yamamoto, K. (Author)
Format: Article
Language:English
Published: Cell Press 2022
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Online Access:View Fulltext in Publisher