Analysis of brain region-specific mRNA synthesis and stability by utilizing adult mouse brain slice culture
Utilization of live animals for mechanistic study is challenging yet pivotal to elucidate pathogenesis of neurological diseases. Here, we present a protocol that employs cultured brain slices derived from adult mice to examine mRNA metabolism. We describe the preparation of acute brain slices and th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cell Press
2022
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |