Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry

Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry

With the growing appreciation of tissue-resident immunity, studying tissue-specific immune cells contributing to both homeostasis and disease is imperative. Here, we provide a protocol for the isolation of human intrahepatic leukocytes (IHL) maximizing viability, purity, and yield. Our protocol is s...

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Bibliographic Details
Main Authors: Aidoo-Micah, G. (Author), Amin, O.E (Author), Burton, A.R (Author), Davies, J. (Author), Easom, N.J (Author), Jeffery-Smith, A. (Author), Kucykowicz, S. (Author), Maini, M.K (Author), Pallett, L.J (Author), Schmidt, N.M (Author), Stegmann, K.A (Author), Swadling, L. (Author), Zakeri, N. (Author)
Format: Article
Language:English
Published: Cell Press 2022
Subjects:
Cell isolation
Flow Cytometry/Mass Cytometry
Health Sciences
Immunology
Single Cell
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