DNA fiber combing protocol using in-house reagents and coverslips to analyze replication fork dynamics in mammalian cells
DNA fiber combing is a versatile technique that provides insight into replication fork dynamics at single-molecule resolution. DNA fibers are bound to silanized coverslips and combed, which straightens and aligns the fibers along a single axis. Here, we present a DNA fiber combing protocol that does...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Cell Press
2022
|
Subjects: | |
Online Access: | View Fulltext in Publisher |