Characterization of ColE1 Production for Robust tolC Plate Dual-Selection in E. coli

Bacterial selection is an indispensable tool for E. coli genetic engineering. Marker genes allow for mutant isolation even at low editing efficiencies. TolC is an especially useful E. coli marker: its presence can be selected for with sodium dodecyl sulfate, while its absence can be selected for wit...

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Bibliographic Details
Main Authors: Baas-Thomas, M.S (Author), Church, G.M (Author), Oehm, S.B (Author), Ostrov, N. (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
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Online Access:View Fulltext in Publisher