Characterization of ColE1 Production for Robust tolC Plate Dual-Selection in E. coli
Bacterial selection is an indispensable tool for E. coli genetic engineering. Marker genes allow for mutant isolation even at low editing efficiencies. TolC is an especially useful E. coli marker: its presence can be selected for with sodium dodecyl sulfate, while its absence can be selected for wit...
Main Authors: | Baas-Thomas, M.S (Author), Church, G.M (Author), Oehm, S.B (Author), Ostrov, N. (Author) |
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Format: | Article |
Language: | English |
Published: |
NLM (Medline)
2022
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Subjects: | |
Online Access: | View Fulltext in Publisher |
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