Noroviruses Surrogate Detection Using Loop-Mediated Isothermal Amplification (LAMP), Conventional RT-PCR and Quantitative RT-PCR in Sg. Tekala and Sg. Gabai Stream Water

Noroviruses Surrogate Detection Using Loop-Mediated Isothermal Amplification (LAMP), Conventional RT-PCR and Quantitative RT-PCR in Sg. Tekala and Sg. Gabai Stream Water

A preliminary study was carried out in order to evaluate a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detecting Noroviruses (NoV) RNA surrogates as external standard for NoV.The detection limit of the NoV RT-LAMP assay was observed to be 22 copies/μL. This RT-LA...

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Bibliographic Details
Main Authors: Ab Wahid, M. (Author), Adnan M.S (Author), Jani, J.M (Author), Khairuddin, D. (Author), Lee Y.Y (Author), Lim A.J.M.S (Author), Mohd Yusof, N.Y (Author), Othman M.H (Author), Rahmat S.N (Author), Zainorizuan M.J (Author)
Format: Article
Language:English
Published: EDP Sciences 2017
Subjects:
Cross reactivity
Environmental engineering
Environmental strain
External standards
Isothermal temperature
Isotherms
Loop mediated isothermal amplifications
Polymerase chain reaction
Quantitative RT-PCR
Reverse transcriptase- polymerase chain reaction
Reverse transcriptases
Rivers
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