Serial ultrathin sections to identify ultrastructural localization of GLUT1 molecules in vesicles in brain endothelial cells-correlative light and electron microscopy in depth

Serial ultrathin sections to identify ultrastructural localization of GLUT1 molecules in vesicles in brain endothelial cells-correlative light and electron microscopy in depth

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Precise immunolocalization of molecules in relation to ultrastructural features is challenging, especially when the target is small and not frequent enough to be included in tiny ultrathin sections randomly selected for electron microscopy (EM). Glucose transporter 1 (GLUT1) is in charge of transpor...

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Bibliographic Details
Main Authors: Kuwahara, H. (Author), Nakamura, A. (Author), Nishida, Y. (Author), Sakamaki, Y. (Author), Uchihara, T. (Author), Wake, K. (Author), Yamada, A. (Author), Yokota, T. (Author)
Format: Article
Language:English
Published: NLM (Medline) 2022
Subjects:
animal
Animals
blood–brain barrier
brain
Brain
CLEM
coated vesicle
electron microscopy
Endothelial Cells
endothelium cell
glucose transporter 1
Glucose Transporter Type 1
GLUT1
Mice
Microscopy, Electron
mouse
osmium tetraoxide
Osmium Tetroxide
pre-embedding method
serial section
ultrastructure
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