MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes

MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes

Genes belonging to the same functional group may include numerous and variable gene sequences, making characterizing and quantifying difficult. Therefore, high-throughput design tools are needed to simultaneously create primers for improved quantification of target genes. We developed MetaFunPrimer,...

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Bibliographic Details
Main Authors: Choi, J. (Author), Cole, J.R (Author), Glanville, K.R (Author), Gunturu, S. (Author), Hall, S.J (Author), Howe, A. (Author), Lee, J. (Author), Liu, J. (Author), McDaniel, M.D (Author), Ouyang, Y. (Author), Tiemann, L.K (Author), Villanueva, P. (Author)
Format: Article
Language:English
Published: American Society for Microbiology 2021
Subjects:
ammonia oxidizing bacterium
Article
Betaproteobacteria
controlled study
cropping system
Environment-specific
Functional gene quantification
Gammaproteobacteria
gene cluster
gene sequence
gene targeting
genome
high throughput sequencing
metagenome
microbial community
Nitrification
nitrogen cycling
Nitrosomonas
Nitrosospira
nonhuman
nucleotide sequence
Primer design
Proteobacteria
Quantitative PCR
real time polymerase chain reaction
RNA 16S
unspecific monooxygenase
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