Assessing multiplex tiling PCR sequencing approaches for detecting genomic variants of SARS-CoV-2 in municipal wastewater

Assessing multiplex tiling PCR sequencing approaches for detecting genomic variants of SARS-CoV-2 in municipal wastewater

Wastewater-based genomic surveillance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus shows promise to complement genomic epidemiology efforts. Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and ra...

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Bibliographic Details
Main Authors: Glier, M. (Author), Kuchinski, K. (Author), Lin, X. (Author), McVea, D. (Author), Mierlo, T.R.-V (Author), Prystajecky, N. (Author), Tyson, J.R (Author), Ziels, R.M (Author)
Format: Article
Language:English
Published: American Society for Microbiology 2021
Subjects:
amplicon
Article
controlled study
coronavirus disease 2019
gene sequence
genetic variability
multiplex reverse transcription polymerase chain reaction
municipal wastewater
nonhuman
real time reverse transcription polymerase chain reaction
RNA degradation
RNA extraction
Severe acute respiratory syndrome coronavirus 2
single nucleotide polymorphism
sludge
variant of concern
viral genomics
whole genome sequencing
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