Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens

Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens

Objectives: Formalin-fixed paraffin-embedded (FFPE) tissue is the standard material for diagnostic pathology but poses relevant hurdles to accurate protein extraction due to cross-linking and chemical alterations. While numerous extraction protocols and chemicals have been described, systematic comp...

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Bibliographic Details
Main Authors: Dressler, F.F (Author), Gemoll, T. (Author), Kiefer, S. (Author), Kirfel, J. (Author), Perner, S. (Author), Revyakina, O. (Author), Schoenfeld, J. (Author), Vogele, D. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2022
Subjects:
Antigen retrieval
Biomarker
Clinical proteomics
Crosslinking reversal
Formalin-fixed paraffin-embedded tissue
Protein analysis
Protein extraction
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