barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection

barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection

Background: Tracking dispersal of microbial populations in the environment requires specific detection methods that discriminate between the target strain and all potential natural and artificial interferents, including previously utilized tester strains. Recent work has shown that genomic insertion...

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Bibliographic Details
Main Authors: Bernhards, C.B (Author), Gibbons, H.S (Author), Goralski, T.D.P (Author), Katoski, S.E (Author), Liem, A.T (Author), Lux, M.W (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
algorithm
Algorithms
article
Bacillus anthracis
Bacillus thuringiensis
Bacillus thuringiensis serovar
Bacteriology
Bar codes
binding site
Binding sites
bioinformatics
Bioinformatics
Bioinformatics tools
biological warfare agent
cross reaction
DNA barcodes
DNA barcoding
DNA Barcoding, Taxonomic
DNA base composition
DNA Primers
forensic science
Genetic barcoding
genetics
genome
Genome
Genome tagging
Identification tags
melting temperature
Microbial forensics
Microbial populations
nonhuman
Polymerase chain reaction
Potential contaminants
primer DNA
qPCR detection
real time polymerase chain reaction
Real-Time Polymerase Chain Reaction
serotype
Specific detection
Tagged strains
target organism
Unique identifications
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