Concatenation of paired-end reads improves taxonomic classification of amplicons for profiling microbial communities

Background: Taxonomic classification of genetic markers for microbiome analysis is affected by the numerous choices made from sample preparation to bioinformatics analysis. Paired-end read merging is routinely used to capture the entire amplicon sequence when the read ends overlap. However, the excl...

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Bibliographic Details
Main Authors: Chain, F.J.J (Author), Dacey, D.P (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
16s
16S
V4
Online Access:View Fulltext in Publisher