cDNA-detector: detection and removal of cDNA contamination in DNA sequencing libraries

cDNA-detector: detection and removal of cDNA contamination in DNA sequencing libraries

Background: Exogenous cDNA introduced into an experimental system, either intentionally or accidentally, can appear as added read coverage over that gene in next-generation sequencing libraries derived from this system. If not properly recognized and managed, this cross-contamination with exogenous...

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Bibliographic Details
Main Authors: Ludwig, L.S (Author), Nayar, U. (Author), Qi, M. (Author), Rheinbay, E. (Author), Wagle, N. (Author)
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Subjects:
cDNA
CDNA
Cloning
complementary DNA
Computational tools
computer simulation
Computer Simulation
Contamination
Cross contamination
'current
DNA sequence
DNA sequences
DNA Sequencing
DNA, Complementary
Experimental system
Gene encoding
gene library
Gene Library
genetics
Genome
Genomics
high throughput sequencing
High-Throughput Nucleotide Sequencing
Libraries
Next-generation sequencing
Quality control
Research results
Sequence Analysis, DNA
Simulation studies
Software
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Internet

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